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Yes, MFA using a Duo Mobile passcode or supported U2F security key while a Windows system is unable to reach Duo's service is supported in version 4.0 and later.

Does Duo Authentication for Windows Logon support offline multifactor authentication?

We recommend first updating any domain controllers with 4.1.0 installed to 4.1.1 before then attempting to install the latest available version of Duo for Windows Logon.

Version 4.1.1, released July 13, 2020, first corrected this issue and is suitable for installation on domain controllers, member servers, and workstations. There was an issue seen with Duo Authentication for Windows Logon and RDversion 4.1.0, on Active Directory domain controllers that may trigger user lockouts. General Are there any issues installing Duo for Windows Logon on Active Directory domain controllers? – Create the registry key and save it anywhere on your PC, double click to run the reg file created, and reboot your PC.Duo integrates with Microsoft Windows client and server operating systems to add two-factor authentication to Remote Desktop and local logons. reg extension and run in order to create the registry keys: In this step, I will be using the registry key as this can also be used to point the server to the Upstream server. Steps: The below syntax should be saved with the. For more information on how-tos surrounding Windows registry settings, see the following link. Handy WSUS Commands( Windows Server Update Services Commands, WAUACLT, PowerShell and USOClient), how to Start, Stop and Restart Windows Server Update Services (WSUS) via PowerShell and CMD, Windows Server Update Services: Windows 2016 Servers does not show up on WSUS console, and WSUS clients appear and disappear from the WSUS Update Services console. The kernel, device drivers, services, Security Accounts Manager, and user interface can all use the registry. The Windows Registry is a hierarchical database that stores low-level settings for the Microsoft Windows operating system and for applications that opt to use the registry.